|
| Status |
Public on Sep 14, 2007 |
| Title |
MPSS-SAP-17bp |
| Sample type |
MPSS |
| |
|
| Source name |
sup/ap1 infloresence - mixed stage, immature buds, signature MPSS
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Plants were grown in soil in growth chamber with 16 hours of light for 5 weeks. Immature inflorescence was harvested ~2 hours after dark. Tissue included inflorescence meristem and early stage floral buds (up to Stage 11/12). Total RNA was isolated using TRIzol reagent.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol reagent
|
| |
|
| Description |
RNA extraction and MPSS experiment in Meyers Lab (http://mpss.udel.edu/)
|
| Data processing |
For each of these samples, we determined the normalized abundance value for each signature in the library in "transcripts per million" or TPM. More details on this process are available in Meyers et al. (2004, Genome Research 14:1641). The numbers in the VALUE column represent the expression level of the corresponding signatures for the SAP library. More details about the samples, library replicates and individual signatures are available at http://mpss.udel.edu/at.
|
| |
|
| Submission date |
Sep 04, 2007 |
| Last update date |
Sep 13, 2007 |
| Contact name |
Junfeng Chen |
| E-mail(s) |
chenj@cs.man.ac.uk
|
| Organization name |
University of Manchester
|
| Department |
School of Computer Science
|
| Street address |
Kilburn Building, Oxford Road
|
| City |
Manchester |
| ZIP/Postal code |
M13 9PL |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL5800 |
| Series (1) |
| GSE8994 |
A Comparison of microarray and MPSS Technology Platforms for Expression Analysis of Arabidopsis |
|
