|
| Status |
Public on May 13, 2016 |
| Title |
MiddleBlastocyst_#1_A |
| Sample type |
SRA |
| |
|
| Source name |
human middle blastocyst
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: middle blastocyst
|
| Treatment protocol |
Oocytes, zygotes and 2-cell-stage embryos were collected at appropriate time based on the embryonic development.
|
| Growth protocol |
The oocytes and embryos for this study were donated from volunteer women with the informed consent signed.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNAs in individual oocyte or embryo were reverse transcribed and amplified using the single RNA-seq method we recently developed.
Libraries were constructed with TruSeq DNA library preparation kit (Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
single cell RNA seq
|
| Data processing |
Illumina CASAVA version 1.8 were used to the basecalling.
Reads were trimmed to remove the adapter sequences and low quality bases.
RNA-seq reads were aligned to the human refference genome (hg19) using Tophat software
Raw read counts and RPKM calculated by HTSeq
Genome_build: hg19
Supplementary_files_format_and_content: txt files reflect RPKM of each gene with Refseq ID.
|
| |
|
| Submission date |
Jul 27, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
yujiao dang |
| E-mail(s) |
1101110321@pku.edu.cn
|
| Organization name |
BIOPIC
|
| Street address |
Yihe road
|
| City |
beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE71318 |
Tracing the expression of circular RNAs in human preimplantation embryos |
|
| Relations |
| BioSample |
SAMN03939218 |
| SRA |
SRX1120865 |
