|
| Status |
Public on Sep 27, 2014 |
| Title |
Seg-1R 24h |
| Sample type |
RNA |
| |
|
| Source name |
human esophageal squamous tissue
|
| Organism |
Homo sapiens |
| Characteristics |
radiation sensitive or not: non-sensitive to radiation
tissue: esophageal
cell line: Seg-1
radiation time point: 24h of radiation
|
| Treatment protocol |
cell irradiation was carried out with a 6MV-X-ray linear accelerator.
|
| Growth protocol |
Cells were cultured in high glucose RPMI 1640 (Invitrogen) supplemented with 10% fetal bovine serum (HyClone) and 50 unit/ml penicillin and 50 µg/ml streptomycin.Cellswere maintained in a humidified incubator with 5% CO2 at 37 oC and split every 3-4 days. Tumor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNA was extracted from each cultured cell line using SV Total RNA Isolation Kit (Promega) according to the manufacturer’s protocol. The extracted RNA was then treated with RNase-Free DNase set (QIAGEN) to remove any contaminating genomic DNA according to the manufacture’s protocol.
|
| Label |
biotin
|
| Label protocol |
Reverse Transcription to Synthesize First Strand cDNA, Second Strand cDNA Synthesis, cDNA Purification, InVitro Transcription to Synthesize biotin-labeled cRNA, cRNA Purification
|
| |
|
| Hybridization protocol |
According to Illumina protocol
|
| Scan protocol |
According to Illumina protocol
|
| Description |
mRNA expression data from non-sensitive to radiation Seg-1 cell line at 24h of radiation
|
| Data processing |
Cubic Spline
|
| |
|
| Submission date |
Sep 22, 2014 |
| Last update date |
Sep 27, 2014 |
| Contact name |
Qingtao Hu |
| E-mail(s) |
huqingtao1987@gmail.com
|
| Organization name |
Beijing Institute of Genomics,Chinese Academy of Sciences
|
| Street address |
NO.1 Beichen West Road, Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE61620 |
Elevated expression of AKR1C3 leads esophageal cancer resistant to ionizing radiation via modulation of oxidative stress |
|
