|
| Status |
Public on Jul 22, 2017 |
| Title |
hasmc_tp0hr_biological rep4 |
| Sample type |
RNA |
| |
|
| Source name |
human aortic smc, control 0hr timepoint, no IL-1beta
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
race: white
tissue: heart
cell type: aortic smooth muscle cell
material type: primary cell
time point: 0
cell line: 3F1243
|
| Biomaterial provider |
Lonza
|
| Treatment protocol |
Cells were incubated with DMSO (vehicle) for 2 hours. Subsequently, cells were stimulated with interleukin-1beta (10 ng/mL). The start of incubation with interleukin-1beta is taken to be time zero.
|
| Growth protocol |
Primary human aortic smooth muscle cells (3F1243) from Lonza were cultured using standard cell culture. Cells were harvested from culture after 0 or 2 hours incubation with interleukin-1beta (10 ng/mL).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen kit was used to isolate total RNA.
|
| Label |
biotin
|
| Label protocol |
Labeling was carried out at the Penn Microarray Facility.
|
| |
|
| Hybridization protocol |
Hybridization was carried out at the Penn Microarray Facility. Affymetrix Human Genome U133A v2 was used.
|
| Scan protocol |
Data were collected at the Penn Microarray Facility.
|
| Data processing |
RMA was used for background correction, normalization and summarization based on a custom CDF: HGU133A2_Hs_ENTREZG_16.0.0
|
| |
|
| Submission date |
Jul 24, 2014 |
| Last update date |
Jul 22, 2017 |
| Contact name |
Emanuela Ricciotti |
| E-mail(s) |
emanuela@mail.med.upenn.edu
|
| Organization name |
University of Pennsylvania
|
| Department |
Department of Pharmacology/Institute for Translational Medicine and Therapeutics
|
| Lab |
FitzGerald Lab
|
| Street address |
3400 Civic Center Blvd.
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL18991 |
| Series (1) |
| GSE59740 |
Human smooth muscle cells at 2 hr post-stimulation with IL-1beta |
|
| Relations |
| Reanalysis of |
GSM1442198 |
