|
| Status |
Public on Jul 26, 2013 |
| Title |
CHOPWT2.2 (DK_B3_15) |
| Sample type |
RNA |
| |
|
| Source name |
wild type hiPS
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Induced pluripotent stem cells
cell line: CHOPWT2.2
|
| Biomaterial provider |
Coriell; http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM05565
|
| Treatment protocol |
iPSCs were enriched by FACS sorted for pluripotency markers SSEA3-FITC (1:100; Biolegend) and Tra-1-81-APC (1:100; Biolegend)
|
| Growth protocol |
Human fibroblast lines CHOPWT1 (Coriell GM00969), CHOPWT2 (Coriell GM05565), and BMC (ATCC CRL-2097) were cultured in IMDM (Mediatech) containing 10% FBS at 37°C and 5% CO2 in a humidified incubator. The iPSCs were maintained in HES media: DMEM/F12 containing 2 mM of glutamine, 20% Knockout Serum Replacement (KO), 1X NEAA, 0.1 mM β-mercaptoethanol, and 10 ng/ml of bFGF (Roche, Indianapolis, IN) on 0.1% gelatin (Sigma, Inc.) coated plates with irradiated mouse embryonic fibroblast (MEFs)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA and miRNA were isolated from cells using the miRNeasy kit (Qiagen, Inc), with the optional column RNAse-free DNase treatment, according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol
|
| |
|
| Hybridization protocol |
Three biological replicates were prepared for all iPSC and hESC-derived samples. Quantified RNA samples were hybridized to Affymetrix GeneChip Human Gene 1.0 ST arrays.
|
| Scan protocol |
Affymetrix protocol
|
| Data processing |
All gene array data obtained from Affymetrix passed quality control steps and were sent to RMA normalization and log transformation by Affymetrix Expression Console.
|
| |
|
| Submission date |
Jul 25, 2013 |
| Last update date |
Aug 13, 2013 |
| Contact name |
Jason Mills |
| E-mail(s) |
millsja@email.chop.edu
|
| Phone |
267-426-0949
|
| Organization name |
The Children's Hospital of Philadelphia
|
| Department |
Laboratory Medicine
|
| Lab |
Center for Cellular and Molecular Therapeutics
|
| Street address |
3501 Civic Center Blvd, CTRB, RM5100
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19004 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE49231 |
Clonal Genetic and Hematopoietic Heterogeneity among Human Induced Pluripotent Stem Cell lines |
|
