|
Status |
Public on Mar 05, 2013 |
Title |
Primary node-positive infiltrating ductal breast carcinoma (D_21T) |
Sample type |
RNA |
|
|
Source name |
Primary node-positive infiltrating ductal breast carcinoma (D_21T)
|
Organism |
Homo sapiens |
Characteristics |
tissue: Primary node-positive infiltrating ductal breast carcinoma
|
Extracted molecule |
total RNA |
Extraction protocol |
For total RNA extraction cryopreserved breast tissue samples were homogenized in TRIzol reagent (Invitrogen, San Diego, CA, USA) using pestle and nuclease-free 1,5ml reaction tubes (Ambion). Total RNA was then extracted following the manufacturers guidelines and quality was assessed with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, USA).
|
Label |
biotin
|
Label protocol |
Standard Affymetrix protocol
|
|
|
Hybridization protocol |
Standard Affymetrix protocol
|
Scan protocol |
Standard Affymetrix protocol using a Gene chip scanner 3000
|
Description |
Primary node-positive infiltrating ductal breast carcinoma (D_21T) sample
|
Data processing |
Data processing cel files were normalized with 'rma' function of the Bioconductor package (v2.10) 'affy' in R.2.15.1
|
|
|
Submission date |
Feb 19, 2013 |
Last update date |
Mar 05, 2013 |
Contact name |
Juanjo Lozano |
E-mail(s) |
juanjo.lozano@ciberehd.org
|
Organization name |
CIBEREHD
|
Department |
Plataforma de Bioinformatica
|
Street address |
C/ Rosselló 153
|
City |
Barcelona |
ZIP/Postal code |
08036 |
Country |
Spain |
|
|
Platform ID |
GPL571 |
Series (1) |
GSE44408 |
Transcriptomic survey of lymph node-positive vs. - negative ductal breast cancer |
|