|
| Status |
Public on Dec 31, 2006 |
| Title |
control nonsmoker 2 |
| Sample type |
RNA |
| |
|
| Source name |
human CD3 positive T cells
|
| Organism |
Homo sapiens |
| Characteristics |
nonsmoker
|
| Treatment protocol |
T -lymphocytes were precipitated untouched from 4ml peripheral blood mononuclear cells, washed in PBS + 2mM EDTA, lysed in TriZol
|
| Extracted molecule |
total RNA |
| Extraction protocol |
the RNA was worked up using PLG Tubes and the Qiagen Rneasy Kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 microg total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG U133A2.0 GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from the non smoking participant 2
|
| Data processing |
The data were analyzed with GCOS 1.4 using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
May 10, 2006 |
| Last update date |
Jun 22, 2006 |
| Contact name |
Sandy Mosig |
| E-mail(s) |
sandy.mosig@mti.uni-jena.de, sandy.mosig@googlemail.com
|
| Phone |
+49-3641-934813
|
| Fax |
+49-3641-933950
|
| Organization name |
University Hospital Jena
|
| Department |
Molecular Hemostaseology - Institute of Vascular Medicine
|
| Street address |
Bachstrasse 18
|
| City |
Jena |
| ZIP/Postal code |
07743 |
| Country |
Germany |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE4806 |
Gene expression profiles of T-lymphocytes are sensitive to the influence of heavy smoking |
|
