|
Status |
Public on Feb 14, 2013 |
Title |
RNA from primary UM 64 |
Sample type |
RNA |
|
|
Source name |
primary uveal melanoma cells
|
Organism |
Homo sapiens |
Characteristics |
cell type: primary uveal melanoma cells gender: F survival (days): 945 death with metastasis: 0 metastasis: 0
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with mirVana kit (Ambion), followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Data processing |
The data were normalised using quantile normalisation with background subtraction.
|
|
|
Submission date |
Feb 13, 2013 |
Last update date |
Feb 14, 2013 |
Contact name |
Li Zhang |
E-mail(s) |
mlizhang@gmail.com
|
Organization name |
CleveLAND CLINIC LERNER COL/MED-CWRU
|
Department |
Quantitative Health Sciences
|
Street address |
9500 Euclid Ave, JJN3
|
City |
Cleveland |
State/province |
OH |
ZIP/Postal code |
44124 |
Country |
USA |
|
|
Platform ID |
GPL6883 |
Series (2) |
GSE44295 |
Genome-wide gene expression analysis of primary uveal melanoma cells, normal uveal melanocytes, neural crest stem cells, embryonic stem cells and uveal melanoma cell lines MUM2B and OCM1A |
GSE44299 |
Primary uveal melanoma cells, normal uveal melanocytes, neural crest stem cells, embryonic stem cells and uveal melanoma cell lines |
|