|
| Status |
Public on Feb 14, 2013 |
| Title |
RNA from primary UM 1 |
| Sample type |
RNA |
| |
|
| Source name |
primary uveal melanoma cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: primary uveal melanoma cells
gender: M
survival (days): 547
death with metastasis: 1
metastasis: 1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with mirVana kit (Ambion), followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with background subtraction.
|
| |
|
| Submission date |
Feb 13, 2013 |
| Last update date |
Feb 14, 2013 |
| Contact name |
Li Zhang |
| E-mail(s) |
mlizhang@gmail.com
|
| Organization name |
CleveLAND CLINIC LERNER COL/MED-CWRU
|
| Department |
Quantitative Health Sciences
|
| Street address |
9500 Euclid Ave, JJN3
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44124 |
| Country |
USA |
| |
|
| Platform ID |
GPL6883 |
| Series (2) |
| GSE44295 |
Genome-wide gene expression analysis of primary uveal melanoma cells, normal uveal melanocytes, neural crest stem cells, embryonic stem cells and uveal melanoma cell lines MUM2B and OCM1A |
| GSE44299 |
Primary uveal melanoma cells, normal uveal melanocytes, neural crest stem cells, embryonic stem cells and uveal melanoma cell lines |
|
