|
| Status |
Public on Apr 01, 2013 |
| Title |
GM19171_EtOH_2h |
| Sample type |
RNA |
| |
|
| Source name |
Lymphoblastoid cell lines
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: EtOH
cell line: GM19171
time: 2h
|
| Biomaterial provider |
Coriell Cell Repositories http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM19171
|
| Treatment protocol |
Cells were treated with either EtOH (vehicle) or 1micromolar dexamethasone
|
| Growth protocol |
LCLs were grown according to standard protocols in RPMI supplemented with 15% charcoal-stripped fetal bovine serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with LUMI in R
|
| |
|
| Submission date |
Feb 11, 2013 |
| Last update date |
Apr 01, 2013 |
| Contact name |
Joseph C Maranville |
| E-mail(s) |
jcmaranville@uchicago.edu
|
| Phone |
7738345239
|
| Organization name |
920 E. 58th Street
|
| Street address |
920 E. 58th Street
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (2) |
| GSE44248 |
Genetic, functional and molecular features of glucocorticoid receptor binding (expression) |
| GSE45640 |
Genetic, functional and molecular features of glucocorticoid receptor binding |
|
