|
| Status |
Public on Jan 01, 2013 |
| Title |
LCL, PA 8 week male, GM22496, low glucose |
| Sample type |
RNA |
| |
|
| Source name |
LCL, low glucose
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
age: 8 wk
disease state: Propionic acidemia
treatment: low glucose
cell line: GM22496
|
| Biomaterial provider |
http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=NA22496
|
| Treatment protocol |
Cells switch to low glucose (RPMI 1640 with no glucose) +15% FBS +2 mM glutamine or RPMI 1640 +15%FBS +2 mM glutamine 24 hours prior to harvest and extraction
|
| Growth protocol |
Cells maintained in RPMI 1640 with 15% FBS, 2 mmol/L L-glutamine, 100 units/mL penicukkub-streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells lysed using Quiashredder and total RNA isolated using Rneasy Purification kits
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were created using GeneChip Whole transcripts sense target Labeling assay (Affymetrix) according to manufacturer's instructions
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Gene 1.0 ST array
|
| Scan protocol |
GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and Primary Component anylsis, Partek
|
| |
|
| Submission date |
Dec 10, 2012 |
| Last update date |
Jan 01, 2013 |
| Contact name |
Kimberly Chapman |
| E-mail(s) |
kchapman@cnmc.org
|
| Phone |
202-476-5278
|
| Organization name |
Children's National Medical Center
|
| Department |
Genetics and Metabolism
|
| Street address |
111 Michigan Ave NW
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20010 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE42816 |
Gene expression propionic patients, carriers, and controls |
|
