|
| Status |
Public on Jun 18, 2013 |
| Title |
MALT1-HSC2, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
HSC2 cells stably transfected MALT1 cDNA
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: oral carcinoma
cell line: HSC2
|
| Growth protocol |
The cells stably expressing full-length wild-type MALT1 (malt1HSC2 cells) and transfected vector alone (mockHSC2 cells) were previously established.They were maintained in 10% fetal bovine serum- and 100 units/ml of penicillin/streptomycin-containing DMEM medium (Sigma-Aldrich, St. Louis, IL) in a conventional 5% CO2 incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 µg of aRNA were hybridized for 16 hr at 45˚C on GeneChip Human Genome U133A 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Data processing |
The data were analyzed with GeneChip Command Console Software (AGCC) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Nov 16, 2012 |
| Last update date |
Jun 18, 2013 |
| Contact name |
Kazushi Imai |
| E-mail(s) |
kimai@tky.ndu.ac.jp
|
| Phone |
81-3-3261-8857
|
| Fax |
81-3-3261-8875
|
| Organization name |
Nippon Dental Universty
|
| Department |
Biochemistry
|
| Street address |
1-9-20 Fujimi, Chiyoda-ku
|
| City |
Tokyo |
| ZIP/Postal code |
102-8159 |
| Country |
Japan |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE42335 |
Expression data for MALT1-responsive genes |
|
