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| Status |
Public on Mar 28, 2017 |
| Title |
Transcriptome analysis on wild type and ddx39a mutant zebrafish embryos by Next Generation Sequencing |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
DEAD box RNA helicase DDX39A has been shown to regulate RNA metabolism; however its role in vertebrate development has not previously been examined. To determine the impact of loss of ddx39a on transcriptome during vertebrate early development, we pursued transcriptome analysis (RNA-Seq) on wild type and ddx39a mutant zebrafish embryos at 24 hour-post-fertilization. And by using RIP-seq to identify targeted RNA which were DDX39A binded.
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| Overall design |
Using RNA-seq to identify the changes in the transcriptomic landscape in ddx39a mutants. Using RIP-seq to identify DDX39A binding RNAs.
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| |
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| Contributor(s) |
Yang Y, Lou X |
| Citation(s) |
29636379 |
| |
| Submission date |
Mar 27, 2017 |
| Last update date |
Nov 08, 2023 |
| Contact name |
Yuxi Yang |
| E-mail(s) |
yuxi.yang@smail.nju.edu.cn
|
| Organization name |
Nanjing University
|
| Department |
Medical School
|
| Lab |
Xin Lou Lab
|
| Street address |
Room 402, 12 Xuefu Road, Nanjing University
|
| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210061 |
| Country |
China |
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| Platforms (1) |
| GPL21741 |
Illumina HiSeq 4000 (Danio rerio) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA380609 |
| SRA |
SRP102513 |
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