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| Status |
Public on Aug 10, 2017 |
| Title |
Impact of HypERrlnc Knockdown on the human pericyte transcriptome |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Pericytes are essential for vessel maturation and endothelial barrier function. Long non-coding RNAs (lncRNAs) regulate endothelial cell function, but their role in pericyte biology remains unexplored. Here we characterize the human pericyte transcriptome following knockdown of lncRNA HypERrlnc (RP11-65J21.3).
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| Overall design |
To investigate the impact of the knockdown of long noncoding RNA HypERrlnc on the pericyte transcriptome. HypERrlnc was silenced by LNA GapmeRs, controls were treated with scramble LNA GapmeR control. HypERrlnc was silenced in 3 independent experiments and total, ribosomal depleted RNA was subsequently analyzed via RNA deep sequencing. Mouse pericytes were kept in pre-equilibrated culture medium in a hypoxic incubator (Labotect) with humidified atmosphere at 5% CO2, 1% O2, 37°C. Mouse Pericytes were subjected to hypoxia for 24 h. Hypoxia was verified with measurement of culture medium pO2 levels with a hypoxia sensing probe (Oxford Optronix) as described elsewhere (Zehendner et al Plos One 2013).
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| Contributor(s) |
Zehendner CM, Dimmeler S |
| Citation(s) |
28611075 |
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| Submission date |
Dec 23, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Christoph Michael Zehendner |
| Organization name |
Goethe University
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| Street address |
Theodor Stern Kai 7
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| City |
Frankfurt am Main |
| ZIP/Postal code |
60590 |
| Country |
Germany |
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| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE92888 |
Long non-coding control of pericyte function |
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| Relations |
| BioProject |
PRJNA358751 |
| SRA |
SRP095640 |
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