|
| Status |
Public on Feb 06, 2018 |
| Title |
Transcriptomic Analysis of Adult Zebrafish Inner Ear Hair Cells |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To understand the basic biological property of hair cells (HCs) from lower vertebrates, we examined transcriptomes of adult zebrafish HCs. GFP-labeled HCs were isolated from the utricle, saccule, and lagena, the three inner-ear sensory epithelia of a pou4f3 promoter-driven GAP-GFP line of transgenic zebrafish. 2,000 HCs and 2,000 non-sensory cells from the inner ear were individually collected by suction pipet technique. RNA sequencing was performed and the resulting sequences were mapped, analyzed, and compared. Comparisons allow us to identify enriched genes in HCs, which may underlie HC specialization.
|
| |
|
| Overall design |
Examination of transcriptomes of adult zebrafish inner ear hair cells and surrounding cells individually collected and sorted using pou4f3 promoter-driven GFP marking hair cells.
|
| Web link |
https://www.nature.com/articles/sdata20185
|
| |
|
| Contributor(s) |
Barta CL, Liu H, Chen L, Giffen KP, Li Y, Kramer KL, Beisel KW, He DZ |
| Citation(s) |
29406519 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 DC004696 |
Biophysics and Development of Cochlear Hair Cells: BIOPHYSICS AND DEVELOPMENT OF COCHLEAR OUTER HAIR CELLS: Biophysics and Development of Cochlear Hair Cells: BIOPHYSICS AND DEVELOPMENT OF COCHLEAR OUTER HAIR CELLS: BIOPHYSICS AND DEVELOPMENT OF COCHLEAR OUTER HAIR CELLS: Biophysics and Development of Cochlear Hair Cells: Biophysics and Development of Cochlear Hair Cells: Biophysics and Development of Cochlear Hair Cells: Biophysics and Development of Cochlear Hair Cells: Biophysics and Development of Cochlear Hair Cells: Biophysics and Development of Cochlear Hair Cells: Biophysics and Development of Cochlear Hair Cells |
CREIGHTON UNIVERSITY |
David Z.Z. He |
|
| |
| Submission date |
Dec 19, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
David Z.Z. He |
| E-mail(s) |
hed@creighton.edu
|
| Phone |
14022801409
|
| Organization name |
Creighton University
|
| Department |
Department of Biomedical Sciences
|
| Street address |
2500 California Plaza
|
| City |
Omaha |
| State/province |
Nebraska |
| ZIP/Postal code |
68178 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
|
| Samples (6)
|
| GSM2432250 |
GFP+ Hair Cells Biological Replicate 1 Technical 1 |
| GSM2432251 |
GFP+ Hair Cells Biological Replicate 1 Technical 2 |
| GSM2432252 |
GFP+ Hair Cells Biological Replicate 2 Technical 1 |
| GSM2432253 |
GFP+ Hair Cells Biological Replicate 2 Technical 2 |
| GSM2432254 |
GFP-neg surrounding cells 1 Technical 1 |
| GSM2432255 |
GFP-neg surrounding cells 1 Technical 2 |
|
| Relations |
| BioProject |
PRJNA358049 |
| SRA |
SRP095338 |
Less...
More...