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| Status |
Public on Feb 09, 2017 |
| Title |
Transcriptional regulation of male-sterility in 7B-1 male-sterile tomato mutant |
| Organism |
Solanum lycopersicum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The 7B-1 tomato (Solanum lycopersicum L. cv Rutgers) is a male-sterile mutant with enhanced tolerance to abiotic stress, which makes it a potential candidate for hybrid seed breeding and stress engineering. Transcriptomic profiles of the 7B-1 male-sterile and wild type (WT) anthers were studied using mRNA sequencing (RNA-Seq). In total, 768 differentially expressed genes (DEGs) were identified, including 132 up-regulated and 636 down-regulated transcripts. Gene ontology (GO) enrichment analysis of DEGs suggested a general impact of the 7B-1 mutation on metabolic processes, such as proteolysis and carbohydrate catabolic process. Sixteen candidates with key roles in regulation of anther development were subjected to further analysis using qRT-PCR and in situ hybridization. Cytological studies showed several defects associated with anther development in the 7B-1 mutant, including unsynchronized anther maturation, dysfunctional meiosis, arrested microspores, defect in callose degradation and abnormal tapetum development. TUNEL assay showed a defect in programmed cell death (PCD) of tapetal cells in 7B-1 anthers. The present study provides insights into the transcriptome of the 7B-1 mutant. We identified several genes with altered expression level in 7B-1 (including beta-1,3 glucanase, GA2oxs, cystatin, cysteine protease, pectinesterase, TA29, and actin) that could potentially regulate anther developmental processes, such as meiosis, tapetum development, and cell-wall formation/degradation.
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| Overall design |
RNA-seq analysis of anthers from wild type and 7B-1 male-sterile mutant tomato plants.
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| Contributor(s) |
Omidvar V, Mohorianu I, Dalmay T, Zheng Y, Fei Z, Claudia A, Mazzucato A, Vecerova V, Sedlarova M, Fellner M |
| Citation(s) |
28178307 |
| Submission date |
Aug 19, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Irina Mohorianu |
| E-mail(s) |
irina.mohorianu@paediatrics.ox.ac.uk
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| Organization name |
University of Oxford
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| Department |
Medical Sciences Division
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| Lab |
Oxford Vaccine Group
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| Street address |
Headington
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| City |
Oxford |
| ZIP/Postal code |
OX3 7LE |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL16345 |
Illumina HiSeq 2000 (Solanum lycopersicum) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA339564 |
| SRA |
SRP082422 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE85859_SLycopersicum_GeneExpressionLevels_DEG.xlsx |
67.5 Kb |
(ftp)(http) |
XLSX |
| GSE85859_SLycopersicum_GeneExpressionLevels_allGenes.xlsx |
2.9 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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