|
| Status |
Public on Jul 31, 2017 |
| Title |
Exposure to mixture of Environmental endocrine disruptors induce miRNAs/isomiRs deregulation in mouse testis |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Purpose: In testis the effects of exposure to mixtures of Endocrine disruptors compounds (EDCs) upon expression of miRNAs were not addressed.
Objective: To identify the expression profiles of the 'miRNome' in mice testis chronic exposed to a defined mixture of five EDCs.
Methods: Pregnant mice from 0.5 post-coital day were exposed in the drinking water to a mixture containing 0.3 mg/Kg-bw/day of each phthalate (DEHP, DBP, BBP), plus 0.05 mg/Kg-bw/day of each alkylphenol (NP, OP) until adulthood of male mouse (60 days old). We characterized the 'miRNome' by next generation sequence (NGS).
Results: In mouse testis exposed to EDCs mixture we detected by NGS 2 up-regulated and 8 down-regulated miRNAs along to 36 isomiRs differentially expressed; these results were validated by RT-qPCR. and functional analysis showed deregulation of testicular hormonal status, spermatogenesis disruption and germ cells apoptosis.
Conclusions: Here we provide the first association between deregulation of miRNAs, isomiRs, with histopathological and hormonal alterations in adult mice testis exposed to mixture of EDCs.
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| Overall design |
Testis snc-RNA profiles of 60-day old mice of control (mice drank water only with the dose of vehicles equivalents diluted) and exposed to EDCs mixture were generated by deep sequency using MiSeq-Illumina sequencer
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| Contributor(s) |
Buñay J, Larriba E, Moreno R, del Mazo J |
| Citation(s) |
28611354 |
| |
| Submission date |
Jul 21, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Julio Buñay Noboa |
| E-mail(s) |
julio.bunay@gmail.com
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| Phone |
+569-77973421
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| Organization name |
Pontificia Univerisidad Catolica de Chile
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| Department |
Physiology
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| Lab |
Endocrinology and reproduccion
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| Street address |
Alameda 340
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| City |
santiago |
| State/province |
Santiago |
| ZIP/Postal code |
8331150 |
| Country |
Chile |
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| Platforms (1) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA330853 |
| SRA |
SRP079224 |
