|
| Status |
Public on Oct 05, 2018 |
| Title |
Intestinal microbiome adjusts the innate immune setpoint during colonization through negative regulation of MyD88 |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Host pathways mediating changes in immune states elicited by intestinal microbial colonization are incompletely characterized. Here we describe alterations of the host immune state induced by colonization of germ-free zebrafish larvae with an intestinal microbial community or single bacterial species. We show that microbiota-induced changes in intestinal leukocyte subsets and whole-body host gene expression are dependent on the innate immune adaptor gene myd88. Similar patterns of gene expression are elicited by colonization with conventional microbiome, as well as mono-colonization with two different zebrafish commensal bacterial strains. By studying loss-of-function myd88 mutants, we find that colonization suppresses Myd88 at the mRNA level. Tlr2 is essential for microbiota-induced effects on myd88 transcription and intestinal immune cell composition.
|
| |
|
| Overall design |
Zebrafish embryos were sterilized to generate germ-free groups. Transcriptomic responses in germ-free embryos were were assessed relative to colonized embryos, either colonized by complex and in characterized microbial communities (Conventionalozation) or by specefic single commensal bacterial species (monoassociation, Exiguobacterium/Chryseobacterium)
|
| Web link |
https://rdcu.be/8yGq
|
| |
|
| Contributor(s) |
Koch BE, Stougaard J, Spaink HP, Yang S, Lamers G |
| Citation(s) |
30291253 |
| |
| Submission date |
Jun 02, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Bjorn Erik Vind Koch |
| E-mail(s) |
b.e.v.koch@biology.leidenuniv.nl
|
| Organization name |
Leiden University
|
| Department |
Institute of Biology
|
| Lab |
MCB
|
| Street address |
Einsteinweg 55
|
| City |
Leiden |
| ZIP/Postal code |
2333 CC |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA324288 |
| SRA |
SRP076050 |
Less...
More...