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| Status |
Public on Aug 13, 2017 |
| Title |
Altered expression of genes in lncRNA-LBCS, hnRNPK and PTBP1 knockdown |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We identify a lncRNA which low expresses and regulates self-renewal of bladder cancer stem cell. So we named it lncRNA-LBCS (low expresses in bladder cancer stem cell). Further study finds that lncRNA-LBCS bind to hnRNPK and PTBP1. To investigate the genes regulated by these, we knockdown lncRNA-LBCS, hnRNPK and PTBP1 by siRNA, respectively, and perform high-throughput sequence. So we identify a series of genes regulated by lncRNA-LBCS, hnRNPK and PTBP1, respectively.
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| Overall design |
Bladder cancer cell line UM-UC-3 was transfected with the contol siRNA or lncRNA-LBCS, hnRNPK and PTBP1 siRNA for 48h, respectively. The RNA of the transfected cells was extracted and sequenced on an Illumina Hiseq 2500 platform and 100 bp paired-end reads were generated.
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| Contributor(s) |
Chen X, Lin T, Huang J |
| Citation(s) |
27862976, 30742945 |
| BioProject |
PRJNA314922 |
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| Submission date |
Apr 01, 2016 |
| Last update date |
Sep 22, 2019 |
| Contact name |
Xu Chen |
| E-mail(s) |
joshua_18chen@163.com
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| Organization name |
Sun Yat-sen Memorial Hospital
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| Department |
Department of Urology
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| Street address |
107. W. Yanjiang Road
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| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510120 |
| Country |
China |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (4)
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| Relations |
| SRA |
SRP071689 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE79832_RAW.tar |
900.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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