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| Status |
Public on Mar 12, 2016 |
| Title |
MicroRNA-124 and -137 cooperativity controls caspase-3 activity through BCL2L13 in hippocampal neural stem cells. [miRNA expression] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
Temporal lobe epilepsy (TLE) can develop from alterations in hippocampal structure and circuit characteristics, and can be modeled in mice by administration of kainic acid (KA). Adult neurogenesis in the dentate gyrus (DG) contributes to hippocampal functions and has been reported to contribute to the development of TLE. Some of the phenotypical changes include neural stem and precursor cells (NPSC) apoptosis, shortly after their birth, before they produce hippocampal neurons.
Here we explored these early phenotypical changes in the DG 3 days after systemic KA administration to mice. Our specific aim was to understand the molecular mechanisms underlying altered apoptosis levels in NSPC following KA-induced status epilepticus (KA-SE). Accordingly, we chose a multi-omics experimental setup and analyzed DG tissue samples using proteomics, transcriptomics and microRNA profiling techniques. We here present a description of how these date were obtained and provide them to others for further analysis and validation. This may help to further identify and characterize molecular mechanisms involved in the alterations induced shortly after KA-SE in the mouse DG.
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| Overall design |
Total RNA obtained from dentate gyrus 72h after mice were subjected to repeated low dose kainic acid induced status epilepticus or saline i.p. injections
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| Contributor(s) |
Schouten M, Bielefeld P, Fratantoni SA, Hubens CJ, Piersma SR, Pham TV, Voskuyl RA, Lucassen PJ, Jimenez CR, Fitzsimons CP |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 11, 2016 |
| Last update date |
Oct 04, 2016 |
| Contact name |
Marijn Schouten |
| E-mail(s) |
mr.m.schouten@gmail.com
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| Organization name |
University of Amsterdam
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| Department |
Swammerdam Institute for Life Sciences
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| Lab |
Fitzsimons
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| Street address |
Science Park 904
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| City |
Amsterdam |
| ZIP/Postal code |
1098XH |
| Country |
Netherlands |
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| Platforms (1) |
| GPL20703 |
Applied Biosystems Taqman Array Rodent MicroRNA CardA v3.0 |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE79132 |
MicroRNA-124 and -137 cooperativity controls caspase-3 activity through BCL2L13 in hippocampal neural stem cells. |
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| Relations |
| BioProject |
PRJNA314997 |
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