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| Status |
Public on Sep 29, 2015 |
| Title |
Mononuclear phagocytes in muscle after notexin injury in C57Bl/6, Cx3cr1-/-, Ccl2-/-,Cx3cr1-/-Ccl2-/- mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Muscle injury triggers inflammation in which infiltrating mononuclear phagocytes are crucial for tissue regeneration. The interaction of the CCL2/CCR2 and CX3CL1/CX3CR1 chemokine axis that guides phagocyte infiltration is incompletely understood. Here, we show that CX3CR1 deficiency promotes muscle repair and rescues Ccl2-/- mice from impaired muscle regeneration as a result of altered macrophage function, not infiltration. Transcriptomic analysis of muscle mononuclear phagocytes reveals that Apolipoprotein E (ApoE) is up-regulated in mice with efficient regeneration. ApoE treatment enhances phagocytosis by mononuclear phagocytes in vitro, and restores phagocytic activity and muscle regeneration in Ccl2-/- mice. Because CX3CR1 deficiency may compensate for defective CCL2-dependant monocyte recruitment by modulating ApoE-dependent macrophage phagocytic activity, targeting CX3CR1 expressed by macrophages might be a powerful therapeutic approach to improve muscle regeneration.
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| Overall design |
Mgl1lo, Mgl1Hi, Ly 6c Hi and F4/80 lo mononuclear phagocyte subsets were isolated from muscles 4 days post Notexin injection; Mgl1lo and Mgl1Hi mononuclear phagocyte subsets were isolated from untreated muscles of 4 mice strains: C57Bl/6 (WT), Cx3cr1-/-, Ccl2-/-,Cx3cr1-/-Ccl2-/- (pool of 3 mice per strain and per condition).
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| Contributor(s) |
Arnold L, Perrin H, Baudesson de Chanville C, Saclier M, Hermand P, Poupel L, Guyon E, Licata F, Carpentier W, Vilar J, Mounier R, Chazaud B, Benhabiles N, Boissonnas A, Combadiere B, Combadiere C |
| Citation(s) |
26632270 |
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| Submission date |
Sep 26, 2015 |
| Last update date |
Oct 09, 2019 |
| Contact name |
Helene Perrin |
| E-mail(s) |
helene.perrin0@upmc.fr
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| Organization name |
INSERM
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| Department |
U945
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| Lab |
B. Combadiere
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| Street address |
91 boulevard de l'hopital
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| City |
Paris |
| ZIP/Postal code |
75013 |
| Country |
France |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA297111 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE73473_RAW.tar |
53.9 Mb |
(http)(custom) |
TAR (of IDAT) |
| GSE73473_non-normalized.txt.gz |
6.8 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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