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| Status |
Public on Sep 14, 2015 |
| Title |
Genome-wide ParB2-DNA and RctB-DNA interaction analyses in Vibrio cholerae |
| Platform organism |
Vibrio cholerae |
| Sample organism |
Vibrio cholerae O1 biovar El Tor str. N16961 |
| Experiment type |
Genome binding/occupancy profiling by array
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| Summary |
Replication and segregation are the two main processes that maintain chromosomes in growing cells. In bacteria, replication and transcription have been proposed to provide motive force in chromosome segregation. Recently, ParB2, a segregation protein, encoded by V. cholerae chromosome II (chrII) was also found to influence chrII replication. V. cholerae chrII replication is primarily controlled by its specific initiator protein, RctB. Here, we have screened for ParB2 and RctB binding sites using a genome-wide DNA binding analysis (ChIP-chip). We report the identification of a new region containing additional RctB and ParB2 binding sites, and suggest the mechanisms to coordinate replication initiation with segregation of chromosomes.
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| Overall design |
ParB2 binding DNA (Cy5) vs total DNA (input, Cy3) in wildtype (CVC209), RctB binding DNA (Cy5) vs total DNA (input, Cy3) in wildtype (CVC209), RctB binding DNA (Cy5) vs total DNA (input, Cy3) in MCH1 (CVC2099, rctB-deleted strain), Biological replicates: 3 or 2
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| Contributor(s) |
Baek J, FitzGerald PC, Chattoraj DK |
| Citation(s) |
23818869, 24586205 |
| Submission date |
Sep 14, 2015 |
| Last update date |
Sep 14, 2015 |
| Contact name |
Jonghwan Baek |
| Organization name |
NIH
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| Department |
NCI
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| Lab |
Laboratory of Biochemistry and Molecular Biology
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| Street address |
37 Convent Dr
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL20909 |
Agilent-026610 Vibrio cholerae Microarray 60K (Feature Number version) |
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| Samples (8)
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| GSM1876191 |
ParB2 binding DNA (Cy5) vs total DNA (input, Cy3) in wildtype_replicate 1 |
| GSM1876192 |
ParB2 binding DNA (Cy5) vs total DNA (input, Cy3) in wildtype_replicate 2 |
| GSM1876193 |
ParB2 binding DNA (Cy5) vs total DNA (input, Cy3) in wildtype_replicate 3 |
| GSM1876194 |
RctB binding DNA (Cy5) vs total DNA (input, Cy3) in wildtype_replicate 1 |
| GSM1876195 |
RctB binding DNA (Cy5) vs total DNA (input, Cy3) in wildtype_replicate 2 |
| GSM1876196 |
RctB binding DNA (Cy5) vs total DNA (input, Cy3) in wildtype_replicate 3 |
| GSM1876197 |
RctB binding DNA (Cy5) vs total DNA (input, Cy3) in MCH1_replicate 1 |
| GSM1876198 |
RctB binding DNA (Cy5) vs total DNA (input, Cy3) in MCH1_replicate 2 |
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| Relations |
| BioProject |
PRJNA295519 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE72978_RAW.tar |
53.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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