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| Status |
Public on Dec 15, 2015 |
| Title |
Spo11-oligo mapping in S. cerevisiae Ctf19/CCAN kinetochore sub-complex mutant mcm21 |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
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| Summary |
During meiosis, crossover recombination is essential to link homologous chromosomes and drive faithful chromosome segregation. Crossover recombination is non-random across the genome, and centromere-proximal crossovers are associated with an increased risk of aneuploidy, including Trisomy 21 in humans. Here, we identify the conserved Ctf19/CCAN kinetochore sub-complex as a major factor that minimizes potentially deleterious centromere-proximal crossovers in budding yeast. We uncover multi-layered suppression of pericentromeric recombination by the Ctf19 complex, operating across distinct chromosomal distances. The Ctf19 complex prevents meiotic DNA break formation, the initiating event of recombination, proximal to the centromere. The Ctf19 complex independently drives the enrichment of cohesin throughout the broader pericentromere to suppress crossovers, but not DNA breaks. This non-canonical role of the kinetochore in defining a chromosome domain that is refractory to crossovers adds a new layer of functionality by which the kinetochore prevents the incidence of chromosome segregation errors that generate aneuploid gametes.
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| Overall design |
Two samples total: two biological replicate Spo11-oligo maps of S. cerevisiae SK1 mcm21 null mutant
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| Contributor(s) |
Vincenten N, Kuhl L, Lam I, Oke A, Kerr A, Hochwagen A, Fung J, Keeney S, Vader G, Marston AL |
| Citation(s) |
26653857 |
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| Submission date |
Sep 03, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Isabel Lam |
| Organization name |
Memorial Sloan Kettering Cancer Center
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| Department |
Molecular Biology
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| Lab |
Keeney Lab
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| Street address |
1275 York Avenue
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| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL17342 |
Illumina HiSeq 2500 (Saccharomyces cerevisiae) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA294661 |
| SRA |
SRP063291 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE72683_RAW.tar |
7.4 Mb |
(http)(custom) |
TAR (of WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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