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| Status |
Public on Jun 27, 2015 |
| Title |
Potential Mechanism of Action of 3'-Demethoxy-6-O-Demethylisoguaiacin on Methicillin-resistant Staphylococcus aureus |
| Organism |
Staphylococcus aureus subsp. aureus MRSA252 |
| Experiment type |
Expression profiling by array
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| Summary |
Methicillin-resistant Staphylococcus aureus is one of the major causative agents associated to infections with a high morbidity and mortality in hospitals worldwide. In previous studies, we reported that lignan 3'-demethoxy-6-O-demethylisoguaiacin isolated and characterized from Larrea tridentata showed the best activity towards methicillin-resistant S. aureus. Understanding of mechanism of action of drugs allows design drugs in a better way. Therefore, we employed microarray to obtain gene expression profile of methicillin-resistant S. aureus after exposure to 3'-demethoxy-6-O-demethylisoguaiacin. The results showed that lignan had an effect on cell membrane affecting proteins of the ATP-binding cassette (ABC) transport system causing bacteria death.
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| Overall design |
This study consisted of comparison of isolated RNA of MRSA not treated and MRSA treated with lignan 3'-demethoxy-6-O-demethylisoguaiacin. Both RNAs samples were differentially dyed with Cy3 and Cy5 during cDNA synthesis and hybridized on DNA chip. Afterwards, the chip was scanned in a GenePix 4000B scanner. The resulting gene expression profile was analyzed in databases for functional annotations to find a potential mechanism of the lignan in MRSA.
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| Contributor(s) |
Favela-Hernández JM, Clemente-Soto AF, Balderas-Rentería I, Garza-González E, Camacho-Corona MR |
| Citation missing |
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| Submission date |
Jun 26, 2015 |
| Last update date |
Jun 27, 2015 |
| Contact name |
Aldo Francisco Clemente soto |
| E-mail(s) |
aldoclemente13@hotmail.com
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| Phone |
8112396162
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| Organization name |
UNIVERSIDAD AUTONOMA DE NUEVO LEÓN
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| Department |
Farmacia
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| Lab |
ingenieria genetica y genomica
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| Street address |
Vicente Guerrero
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| City |
Monterrey |
| State/province |
Nuevo Leon |
| ZIP/Postal code |
64570 |
| Country |
Mexico |
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| Platforms (1) |
| GPL20315 |
MYcroarray CAT Staphylococcus aureus subsp. aureus MRSA252 30K 4rep V1 |
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| Samples (1) |
| GSM1723540 |
Treatment with 3'-demethoxy-6-O-demethylisoguaiacin |
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| Relations |
| BioProject |
PRJNA288293 |
