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| Status |
Public on Oct 24, 2016 |
| Title |
Long non-coding RNA produced by RNA polymerase V determines boundaries of heterochromatin |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report genome-wide detection of long noncoding RNA (lncRNA) generate by Pol V in transcriptional gene silencing in Arabidopsis thaliana. We further show that most of these transcripts are bound by AGO4.
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| Overall design |
RNA immunoprecipitation followed by high-throughput sequencing (RIP-seq)
RNA immunoprecipitation with an anti-AGO4 antibody was performed in one biological replicate and included Col-0, nrpe1, and ago4-1; RNA immunoprecipitation with an anti-NRPE1 (largest subunit of Pol V) was performed in two replicates; replicate 1 included Col-0 and nrpe1, replicated two included Col-0, nrpe1, ago4-1, and idn2-1
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| Contributor(s) |
Böhmdorfer G, Sethuraman S, Rowley MJ, Krzysztoń M, Rothi MH, Bouzit L, Wierzbicki AT |
| Citation(s) |
27779094 |
| |
| Submission date |
Jun 25, 2015 |
| Last update date |
Jul 30, 2019 |
| Contact name |
Andrzej T Wierzbicki |
| E-mail(s) |
wierzbic@umich.edu
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| Organization name |
University of Michigan
|
| Department |
Molecular, Cellular, and Developmental Biology
|
| Lab |
Wierzbicki lab
|
| Street address |
830 N. University
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
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| Platforms (1) |
| GPL17639 |
Illumina HiSeq 2500 (Arabidopsis thaliana) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA288172 |
| SRA |
SRP059890 |
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