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| Status |
Public on Jan 25, 2016 |
| Title |
Histone variant 3 regulates RNA polymerase II transcription termination and dual strand transcription of siRNA loci in Trypanosoma brucei |
| Organism |
Trypanosoma brucei |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Histone variant 3 (H3V) is a kinetoplastid specific histone variant that is enriched at RNA polymerase II termination sites and telomeres. We previously found that the DNA modification base J, which co-localizes with H3V, functions to prevent readthrough transcription within gene clusters in T. brucei. We now demonstrate a similar role for H3V, where the loss of this histone variant results in increased expression of downstream genes. The effect is larger when both H3V and J are lost. Additionally, removal of H3V results in increased siRNAs from dual strand transcribed loci as well as increased expression of silent VSGs, indicating a role of H3V in the regulation of antigenic variation
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| Overall design |
The role of H3V in regulating transcription termination was investigated using WT and H3V KO T. brucei cell lines. The role of J was also studied by treating each of these cell lines with DMOG, an inhibitor of J synthesis. We used 4 RNA seq libraries to determine gene expression changes following the loss of H3V and J
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| Contributor(s) |
Sabatini R, Reynolds D, Cliffe L, Siegel N, Alabady M, Hofmeister B, Schmitz RJ |
| Citation(s) |
26796527 |
| |
| Submission date |
Jun 16, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Robert J Schmitz |
| E-mail(s) |
schmitz@uga.edu
|
| Organization name |
University of Georgia
|
| Department |
Genetics
|
| Street address |
B416 Davison Life Sciences
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30602 |
| Country |
USA |
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| Platforms (1) |
| GPL20572 |
Illumina NextSeq 500 (Trypanosoma brucei) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA287144 |
| SRA |
SRP059552 |
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