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| Status |
Public on Nov 01, 2015 |
| Title |
Gene expression profiling of E13.5 Eda null embryonic mammary buds |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
TNF ligand ectodysplasin-A1 contributes to embryonic mammary gland development. We searched for target genes of the Eda pathway using profiling of genes differentially expressed in Eda-null mammary buds after a short exposure to recombinant Fc-Eda-A1 protein.
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| Overall design |
Microdissected E13.5 Eda-/- epithelial mammary buds from 5-6 embryos were pooled together. Other half of an embryo was used as a control and the other half as a treated sample. Littermates from 3 litters of B6CBA background were used for the analysis. Array and data analysis were performed in the Biomedicum Functional Genomics Unit (University of Helsinki, Finland).
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| Contributor(s) |
Lindfors P, Voutilainen M, Mikkola M |
| Citation(s) |
26581094 |
| |
| Submission date |
Jun 11, 2015 |
| Last update date |
Jul 31, 2019 |
| Contact name |
Maria Voutilainen |
| E-mail(s) |
maria.voutilainen@helsinki.fi
|
| Organization name |
University of Helsinki
|
| Department |
Institute of Biotechnology
|
| Lab |
Marja Mikkola
|
| Street address |
Viikinkaari 9
|
| City |
Helsinki |
| ZIP/Postal code |
00014 |
| Country |
Finland |
| |
|
| Platforms (1) |
| GPL20316 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [Ver. 14.1.0, MoEx10stv1_Mm_ENSG] |
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| Samples (6)
|
| GSM1708700 |
Non-treated control, Eda null E13.5 mammary buds, biological replicate 1 |
| GSM1708701 |
Fc-Eda-A1 treated (4hours), Eda null E13.5 mammary buds, biological replicate 1 |
| GSM1708702 |
Non-treated control, Eda null E13.5 mammary buds, biological replicate 2 |
| GSM1708703 |
Fc-Eda-A1 treated (4hours), Eda null E13.5 mammary buds, biological replicate 2 |
| GSM1708704 |
Non-treated control, Eda null E13.5 mammary buds, biological replicate 3 |
| GSM1708705 |
Fc-Eda-A1 treated (4hours), Eda null E13.5 mammary buds, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA286767 |
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