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| Status |
Public on Oct 07, 2015 |
| Title |
Preimplantation stage-specific effect of culture environment on the DNA methylome of bovine blastocysts |
| Organism |
Bos taurus |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
The culture conditions of the early preimplantation embryos affects the DNA methylation landscape of the resulting blastocysts.
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| Overall design |
Five groups of blastocysts were generated. The first (ZY), second (4C) and third (16C) blastocysts groups were obtained after transfer of in vitro developed zygote, 4-cell and 16-cell stage embryos, respectively to synchronized Simmental heifers followed by blastocysts recovery at day 7 of the estrous cycle. The fourth (IVP) and the fifth (VO) blastocyst groups represent those produced completely under in vitro and in vivo culture conditions, respectively. The global DNA methylation pattern of ZY, 4C, 16C and IVP blastocyst groups was investigated with reference to the VO group using EDMA array.
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| Contributor(s) |
Salilew-Wondim D, Fournier E, Hoelker M, Saeed-Zidane M, Tholen E, Looft C, Neuhoff C, Besenfelder U, Havlicek V, Rings F, Gagné D, Sirard M, Robert C, Gad A, Schellander K, Tesfaye D |
| Citation(s) |
26536655 |
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| Submission date |
May 22, 2015 |
| Last update date |
Jan 06, 2016 |
| Contact name |
Dessie Salilew-Wondim |
| E-mail(s) |
dsalilew@uni-bonn.de
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| Organization name |
University of Bonn
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| Department |
Animal breeding and husbandry group
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| Street address |
Endenicher Allee 15
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| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
D-53115 |
| Country |
Germany |
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| Platforms (1) |
| GPL18384 |
EmbryoGENE DNA Methylation Array Bovine 2x400K v2.0 |
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| Samples (16)
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| GSM1694643 |
Blastocysts from in vitro zygote transfer vs. in vivo blastocysts-replicate 1 |
| GSM1694644 |
Blastocysts from in vitro zygote transfer vs. in vivo blastocysts-replicate 2 |
| GSM1694645 |
Blastocysts from in vitro zygote transfer vs. in vivo blastocysts-replicate 3 |
| GSM1694646 |
Blastocysts from in vitro zygote transfer vs. in vivo blastocysts-replicate 4 |
| GSM1694647 |
Blastocysts from in vitro 4 cell transfer vs. in vivo blastocysts-replicate 1 |
| GSM1694648 |
Blastocysts from in vitro 4 cell transfer vs. in vivo blastocysts-replicate 2 |
| GSM1694649 |
Blastocysts from in vitro 4 cell transfer vs. in vivo blastocysts-replicate 3 |
| GSM1694650 |
Blastocysts from in vitro 4 cell transfer vs. in vivo blastocysts-replicate 4 |
| GSM1694651 |
Blastocysts from in vitro 16 cell transfer vs. in vivo blastocysts- replicate 1 |
| GSM1694652 |
Blastocysts from in vitro 16 cell transfer vs. in vivo blastocysts- replicate 2 |
| GSM1694653 |
Blastocysts from in vitro 16 cell transfer vs. in vivo blastocysts- replicate 3 |
| GSM1694654 |
Blastocysts from in vitro 16 cell transfer vs. in vivo blastocysts- replicate 4 |
| GSM1694655 |
Blastocysts from complete in vitro culture vs. in vivo blastocysts-replicate 1 |
| GSM1694656 |
Blastocysts from complete in vitro culture vs. in vivo blastocysts-replicate 2 |
| GSM1694657 |
Blastocysts from complete in vitro culture vs. in vivo blastocysts-replicate 3 |
| GSM1694658 |
Blastocysts from complete in vitro culture vs. in vivo blastocysts-replicate 4 |
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| Relations |
| BioProject |
PRJNA284748 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE69173_Normalized_data_with_controls.txt.gz |
35.3 Mb |
(ftp)(http) |
TXT |
| GSE69173_RAW.tar |
459.0 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
| Processed data are available on Series record |
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