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| Status |
Public on Jul 09, 2015 |
| Title |
Next Generation Sequencing of tomato WT, M82 and pro∆GRAS mutant treated with Gibberellin |
| Organism |
Solanum lycopersicum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of this study was to search for DELLA-independent GA-regulated genes in tomato. RNA deep sequencing (RNA-seq) was performed to GA-treated M82 and pro∆GRAS mutant. Using a two fold increased or decreased cutoff we identified 81 GA-up regulated and 15 GA-down regulated genes, from which five genes were DELLA-independent.
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| Overall design |
M82 and pro∆GRAS seedlings were treated with Paclobutrazol (10mg/l) once a day for three days followed by a single GA3 application (100 µM). Three hours after the GA treatment, young leaves were collected and RNA was extracted for Illumina HiSeq 2500 sequencing. A total of eight samples were analyzed, each treatment had two biological replicates.
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| Contributor(s) |
Livne S, Lor VS, Nir I, Eliaz N, Aharoni A, Olszewski N, Eshed Y, Weiss D |
| Citation(s) |
26036254 |
| Submission date |
Apr 17, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Gilgi Friedlander |
| Organization name |
Weizmann Institute of Science
|
| Street address |
Hertzel st
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
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| Platforms (1) |
| GPL19694 |
Illumina HiSeq 2500 (Solanum lycopersicum) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA281504 |
| SRA |
SRP057396 |
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