|
| Status |
Public on Jul 07, 2015 |
| Title |
Parent-of-Origin Allelic Expression in the Mouse Cerebellum |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The maternal and paternal genomes play different roles in mammalian brains as a result of genomic imprinting, an epigenetic regulation leading to differential expression of the parental alleles of some genes. Here we investigate genomic imprinting in the cerebellum using a newly developed Bayesian statistical model that provides unprecedented transcript-level resolution. We uncover 160 imprinted transcripts, including 41 novel and independently validated imprinted genes. Strikingly, many genes exhibit parentally biased -rather than monoallelic- expression, with different magnitudes according to age, organ, and brain region. Developmental changes in parental bias and overall gene expression are strongly correlated, suggesting combined roles in regulating gene dosage. These findings reveal the remarkable complexity of genomic imprinting, with important implications for understanding the normal and diseased brain.
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| Overall design |
48 samples of F1 mouse hybrids produced by crossing C57Bl/6J males with Cast/EiJ females (denoted as F1i) and reciprocally crossing Cast/EiJ males and C57Bl/6J famales (denoted as F1r).
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| |
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| Contributor(s) |
Perez JD, Rubinstein ND, Fernandez DE, Dulac C |
| Citation(s) |
26140685 |
| |
| Submission date |
Apr 03, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Nimrod Daniel Rubinstein |
| Organization name |
Harvard University
|
| Department |
Molecular and Cellular Biology
|
| Lab |
Dulac
|
| Street address |
16 Divinity Avenue
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA280291 |
| SRA |
SRP056865 |
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