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| Status |
Public on Jun 19, 2017 |
| Title |
Elevated acid ceramidase promotes acute myeloid leukemia blast survival through sphingolipid dysregulation and Mcl-1 upregulation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression of patient samples with acute myeloid leukemia (AML) were compared to normal controls to study dysregulated signalling pathways. Peripheral blood mononuclear cells (PBMCs) from primary AML patient samples were isolated using the Ficoll-Paque gradient separation method. RNA from CD34+ bone marrow cells of healthy donors were purchased from AllCells LLC (Alameda, CA; catalog number RNA-BM003C). Total RNA were harvested from patient samples using Trizol and subjected to microarray-based gene expression analysis.
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| Overall design |
Total RNA from patient PBMCs and healthy donor CD34+ bone marrow cells.
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| Contributor(s) |
Tan SF, Barth BM, Kester M, Cabot MC, Keasey N, Liu X, Shah M, Claxton DF, Feith DJ, Fox TE, Broeg K, Wang HG, Loughran TP Jr |
| Citation(s) |
27825124 |
| Submission date |
Jan 28, 2015 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Stephen Turner |
| Organization name |
Signature Science, LLC
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| Street address |
1670 Discovery Drive
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| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22911 |
| Country |
USA |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (38)
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| Relations |
| BioProject |
PRJNA273897 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE65409_RAW.tar |
6.2 Mb |
(http)(custom) |
TAR |
| GSE65409_non-normalized.txt.gz |
7.4 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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