|
| Status |
Public on Jan 07, 2015 |
| Title |
Expression data from RhoA-null and wildtype keratinocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Using mice with a keratinocyte-restricted deletion of the RhoA gene, we explored its role in the regulation of gene expression in vivo and in vitro
|
| |
|
| Overall design |
RNA was isolated from RhoA-null or wildtype keratinocytes, in two different conditions, freshly isolated (in vivo) or cultured (in vitro), using the RNeasy kit (Qiagen, Germany). Hybridization to GeneChip Mouse Genome 430 2.0 Array (Affymetrix, Santa Clara, CA) was carried out at the Copenhagen University Hospital Microarray Center using standard protocols. 2 biological replicates were analyzed for each in vivo genotype (2 samples from RhoA-null, 2 from wildtype), while 3 replicates for each in vitro genotype.
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| |
|
| Contributor(s) |
Peyrollier K, Mariscal AG |
| Citation(s) |
27754752 |
| |
| Submission date |
Jan 06, 2015 |
| Last update date |
May 17, 2019 |
| Contact name |
Alberto Garcia Mariscal |
| E-mail(s) |
aga@bric.ku.dk
|
| Organization name |
University of Copenhagen
|
| Department |
BRIC
|
| Lab |
Brakebusch Lab
|
| Street address |
Ole Maaløes Vej 5
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (10)
|
| GSM1577765 |
freshly isolated Wildtype in vivo biological rep1 |
| GSM1577766 |
freshly isolated Wildtype in vivo biological rep2 |
| GSM1577767 |
freshly isolated RhoA-KO in vivo biological rep1 |
| GSM1577768 |
freshly isolated RhoA-KO in vivo biological rep2 |
| GSM1577769 |
cultured primary Wildtype in vitro biological rep1 |
| GSM1577770 |
cultured primary Wildtype in vitro biological rep2 |
| GSM1577771 |
cultured primary Wildtype in vitro biological rep3 |
| GSM1577772 |
cultured primary RhoA-KO in vitro biological rep1 |
| GSM1577773 |
cultured primary RhoA-KO in vitro biological rep2 |
| GSM1577774 |
cultured primary RhoA-KO in vitro biological rep3 |
|
| Relations |
| BioProject |
PRJNA271709 |
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