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Series GSE64570 Query DataSets for GSE64570
Status Public on Nov 18, 2015
Title Gene expression profiling of zebrafish embryos at 1 hour post injection of PAMPs
Organism Danio rerio
Experiment type Expression profiling by high throughput sequencing
Summary We use the zebrafish embryo model to study the transcriptome responses to flagellin and Pam3CSK4. Therefore, we injected these PAMPs into the caudal vein at the 27 hours post fertilization and took samples at 1 hour post injection.
 
Overall design This deep sequence study was designed to determine the gene expression profile by Pam3CSK4 and flagellin injection. RNA was isolated from embryos at 1 hour post injection. Wildtypes and tlr2- and tlr5a- morphants zebrafish embryos were micro-injected into the caudal vein with 1ng of Pam3CSK4, 0,1 ng flagellin , or water as a control at 27 hours post fertilization. After injections embryos were transferred into fresh egg water and incubated at 28°C. At 1 hour post injection triplicates of 10 to 15 embryos per condition were snap-frozen in liquid nitrogen, and total RNA was isolated using TRIZOL reagent.
 
Contributor(s) Yang S, Marín-Juez R, Spaink H
Citation(s) 26208853, 30577878
Submission date Dec 30, 2014
Last update date May 15, 2019
Contact name shuxin yang
E-mail(s) s.yang@biology.leidenuniv.nl
Phone 0031633380337
Organization name Leiden University
Street address Einsteinweg 55
City Leiden
ZIP/Postal code 2333 CC
Country Netherlands
 
Platforms (1)
GPL18413 Illumina HiSeq 2500 (Danio rerio)
Samples (21)
GSM1574496 Wildtype: water-injected control; Biological replica 1
GSM1574497 Wildtype: water-injected control; Biological replica 2
GSM1574498 Wildtype: water-injected control; Biological replica 3
Relations
BioProject PRJNA271342
SRA SRP051621

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64570_RAW.tar 22.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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