|
| Status |
Public on Apr 27, 2015 |
| Title |
FCRL5 delineates functionally impaired memory B cells associated with malaria exposure |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Classical and atypical memory B cells from Ugandan children were compared in their baseline gene expression patterns.
|
| |
|
| Overall design |
Independent cryopreserved PBMC samples from children were thawed, and classical and atypical memory B cells were double sorted by FACS directly into an RNA lysis buffer. RNA was isolated, amplified using 2 rounds of linear amplification, and hybridized to Agilent-039494 Human 8x60k microarrays as a single color experiment.
|
| |
|
| Contributor(s) |
Kim CC, Sullivan R, Greenhouse B |
| Citation(s) |
25993340 |
| |
| Submission date |
Dec 23, 2014 |
| Last update date |
Mar 27, 2018 |
| Contact name |
Charlie Kim |
| E-mail(s) |
cckim47@gmail.com
|
| Organization name |
University of California, San Francisco
|
| Department |
Medicine
|
| Lab |
Kim
|
| Street address |
1001 Potrero Ave
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94110 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16699 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Feature Number version) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA271107 |
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