|
| Status |
Public on Feb 01, 2004 |
| Title |
Erythroid Differentiation: G1E Model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
G1E cells are a Gata-1 erythroid-committed cell line derived from targeted disruption of Gata-1 in embryonic stem cells. The ER4 subclone contains an inducible form of Gata-1 (Gata-1-ER, Gata-1 fused to the estradiol receptor ligand binding domain). We performed transcriptome analysis using this cell line. Estradiol was added to culture medium triggering synchronous and homogenous differentiation. At various time points, RNA was sampled and analyzed using the Affymetrix MG-U74Av2 platform. Three biological replicas (A,B, and C) were performed. The thirty hour time course corresponds to development from the late BFU-E stage through the orthochromatic erythroblast stage.
Keywords = Gata1
Keywords = erythroid
Keywords = mouse
Keywords: time-course
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| Contributor(s) |
Welch JJ, Chodosh LA, Weiss MJ |
| Citation(s) |
15297311 |
| |
| Submission date |
Sep 04, 2003 |
| Last update date |
Feb 18, 2018 |
| Contact name |
John J. Welch |
| E-mail(s) |
welchjo@email.chop.edu
|
| Phone |
215-590-0558
|
| Fax |
215-590-4834
|
| Organization name |
Children's Hospital of Philadelphia
|
| Department |
Division of Hematology
|
| Lab |
Weiss Laboratory
|
| Street address |
34th & Civic Center Blvd.
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
|
| Samples (18)
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| Relations |
| BioProject |
PRJNA87533 |
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