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| Status |
Public on Apr 10, 2015 |
| Title |
Transcriptional profile of the head of zebrafish deiodinase knockdown |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
The role of deiodinase-mediated TH effects in early vertebrate development is only partially understood. Therefore, we investigated the role of deiodinases during early development of zebrafish until 96 hours post fertilization at the level of the transcriptome, biochemistry, morphology and physiology using morpholino (MO) knockdown. Knockdown of D1+D2 (D1D2MO) and knockdown of D3 (D3MO) both resulted in transcriptional regulation of metabolism and (muscle) development in abdomen and tail, together with reduced growth, impaired swim bladder inflation, reduced protein content and reduced motility. The reduced growth and impaired swim bladder inflation in D1D2MO could be due to lower levels of T3 which is known to drive growth and development. The pronounced upregulation of a large number of transcripts coding for key proteins in ATP-producing pathways in D1D2MO could reflect a compensatory response to a decreased metabolic rate, also typically linked to hypothyroidy. Compared to D1D2MO, the effects were more pronounced or more frequent in D3MO, in which hyperthyroidy is expected. More specifically, increased heart rate, delayed hatching and increased carbohydrate content were observed only in D3MO. An increase of the metabolic rate, a decrease of the metabolic efficiency and a stimulation of gluconeogenesis using amino acids as substrates may have been involved in the observed reduced protein content, growth and motility in D3MO larvae. Furthermore, expression of transcripts involved in eye development and phototransduction was decreased in both knockdown conditions, suggesting that both may impair eye development and function. This study provides new insights, not only into the role of deiodinases, but also into the importance of a correct TH balance during vertebrate embryonic development.
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| Overall design |
A-optimal design. Experimental design, D1D2MO vs SCMO and D3MO vs SCMO. Biological replicates: 4 SCMO, 4 D1D2MO, 4 D3MO
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| Contributor(s) |
Bagci E, Heijlen M, Vergauwen L, Hagenaars A, Houbrechts AM, Esguerra CV, Blust R, Darras VM, Knapen D |
| Citation(s) |
25855985 |
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| Submission date |
Sep 22, 2014 |
| Last update date |
Dec 17, 2015 |
| Contact name |
Lucia Vergauwen |
| E-mail(s) |
lucia.vergauwen@uantwerpen.be
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| Phone |
003232652791
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| Organization name |
University of Antwerp
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| Department |
Veterinary Sciences
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| Lab |
Zebrafishlab
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| Street address |
Universiteitsplein 1
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| City |
Wilrijk |
| ZIP/Postal code |
2160 |
| Country |
Belgium |
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| Platforms (1) |
| GPL14664 |
Agilent-026437 D. rerio (Zebrafish) Oligo Microarray V3 (Probe Name version) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE61625 |
Transcriptional profile of deiodinase knockdown in zebrafish head, abdomen and tail |
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| Relations |
| BioProject |
PRJNA261713 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE61622_RAW.tar |
95.7 Mb |
(http)(custom) |
TAR (of GPR) |
| GSE61622_diffgenes.D1D2MO_vs_SCMO_head.txt.gz |
8.7 Kb |
(ftp)(http) |
TXT |
| GSE61622_diffgenes.D3MO_vs_D1D2MO_head.txt.gz |
14.7 Kb |
(ftp)(http) |
TXT |
| GSE61622_diffgenes.D3MO_vs_SCMO_head.txt.gz |
33.4 Kb |
(ftp)(http) |
TXT |
| GSE61622_genes.all.contrasts_head.txt.gz |
682.5 Kb |
(ftp)(http) |
TXT |
| Processed data are available on Series record |
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