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| Status |
Public on Nov 04, 2014 |
| Title |
Expression data from BCL-3 overexpression HaCat cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Constitutive activation of EGFR- and NF-kB-dependent pathways is a hallmark of cancer, yet signaling proteins that connect both oncogenic cascades are poorly characterized. Here we define KIAA1199 as a BCL-3- and p65-dependent gene in transformed keratinocytes. KIAA1199 expression is enhanced upon human papillomavirus (HPV) infection and is aberrantly expressed in clinical cases of cervical (pre)neoplastic lesions. Mechanistically, KIAA1199 binds Plexin A2 and protects from Semaphorin 3A-mediated cell death by promoting EGFR stability and signaling. Moreover, KIAA1199 is an EGFR-binding protein and KIAA1199 deficiency impairs EGF-dependent Src, MEK1 and ERK1/2 phosphorylations. Therefore, EGFR stability and signaling to downstream kinases requires KIAA1199. As such, KIAA1199 promotes EGF-mediated epithelial-mesenchymal transition (EMT). Taken together, our data define KIAA1199 as an oncogenic protein induced by HPV infection and constitutive NF-kB activity that transmits pro-survival and invasive signals through EGFR signaling.
We used microarrays to detail the global programme of gene expression upon BCL-3 overexpression
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| Overall design |
We used two experimental conditions, namely HaCat cells infected with a control lentivirus as well as HaCat cells infected with a BCL-3 expressing construct. Both experimental conditions were in triplicates.
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| Contributor(s) |
Zhang X, Hennuy B, Chariot A |
| Citation(s) |
25366117 |
| Submission date |
Aug 20, 2014 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Alain Chariot |
| E-mail(s) |
alain.chariot@ulg.ac.be
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| Phone |
003243662548
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| Organization name |
University of Liege
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| Lab |
Medical Chemistry
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| Street address |
Avenue de l'hopital
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| City |
Liege |
| State/province |
Liege |
| ZIP/Postal code |
4000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA258529 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE60551_RAW.tar |
13.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| GSE60551_p-values.xls.gz |
13.4 Kb |
(ftp)(http) |
XLS |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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