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| Status |
Public on Oct 01, 2014 |
| Title |
Profiling microRNAs in Eucalyptus grandis reveals no mutual relationship between alterations in miR156 and miR172 expression and adventitious root induction during development |
| Organism |
Eucalyptus grandis |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Background: The change from juvenile to mature phase in woody plants is often accompanied by a gradual loss of rooting ability, as well as by reduced microRNA (miR) 156 and increased miR172 expression.
Results: We characterized the population of miRNAs of Eucalyptus grandis and compared by Northern blot the gradual reduction in miR156 and increase in miR172 expression during development to the loss of rooting ability. Forty known and eight novel miRNAs were discovered and their predicted targets are listed. The expression pattern of nine miRNAs was determined during adventitious root formation in juvenile and mature cuttings. While the expression levels of miR156 and miR172 were inverse in juvenile and mature tissues, no mutual relationship was found between high miR156 expression and rooting ability, or high miR172 expression and loss of rooting ability. This is shown both in E. grandis and also in E. brachyphylla, in which explants that underwent rejuvenation in tissue culture conditions were also examined.
Conclusions: It is suggested that in these Eucalyptus species, there is no correlation between the switch of miR156 with miR172 expression in the stems and the loss of rooting ability.
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| Overall design |
Examination of microRNA in seedlings of Eucalyptus grandis
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| Contributor(s) |
Levy A, Szwerdszarf D, Abu-Abied M, Mordehaev I, Yaniv Y, Riov J, Arazi T, Sadot E |
| Citation(s) |
24965948 |
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| Submission date |
Jun 10, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Tzahi Arazi |
| E-mail(s) |
tarazi@agri.gov.il
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| Phone |
972-3-9683498
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| Organization name |
ARO - Volcani Center
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| Department |
Institute of Plant Sciences
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| Lab |
Tzahi Arazi
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| Street address |
Institute of Plant Sciences, ARO the Volcani Center
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| City |
Bet Dagan |
| State/province |
ISRAEL |
| ZIP/Postal code |
50250 |
| Country |
Israel |
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| Platforms (1) |
| GPL18777 |
Illumina HiSeq 2000 (Eucalyptus grandis) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA252263 |
| SRA |
SRP043079 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE58367_RAW.tar |
480.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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