|
| Status |
Public on Feb 28, 2015 |
| Title |
Scramble and Brown fat lncRNA 1 knockdown (shBlnc1) expressing differentiated brown adipocyte |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Blnc1 is a novel nuclear lncRNA that promotes brown and beige adipocyte differentiation and function. Blnc1 forms a ribonucleoprotein complex with transcription factor EBF2 to stimulate the thermogenic gene program. Further, Blnc1 itself is a target of EBF2, thereby forming a feedforward regulatory loop to drive adipogenesis toward thermogenic phenotype.
We used microarrays to elucidate the role of Blnc1 on brown adipocyte differentiation and mitochondrial function.
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| Overall design |
Brown adipocytes expressing Scramble or brown fat lncRNA 1 knockdown (shBlnc1) were differentiated for 6 days and harvested for RNA isolation and microarray using Affymetrix Mouse MG-430 PM Strip arrays. Two replicated samples were included in this study.
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| |
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| Contributor(s) |
Zhao X, Li S, Wang G, Yu Q, Lin JD |
| Citation(s) |
25002143 |
| |
| Submission date |
May 12, 2014 |
| Last update date |
Aug 06, 2018 |
| Contact name |
xuyun zhao |
| E-mail(s) |
zhaobig_8888@hotmail.com
|
| Organization name |
Life Sciences Institute
|
| Street address |
210 washtenaw ave
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
|
| Samples (4)
|
| GSM1384356 |
Scramble expressing brown adipocytes, biological rep1 |
| GSM1384357 |
Scramble expressing brown adipocytes, biological rep2 |
| GSM1384358 |
Blnc1 shRNA expressing brown adipocytes, biological rep1 |
| GSM1384359 |
Blnc1 shRNA expressing brown adipocytes, biological rep2 |
|
| Relations |
| BioProject |
PRJNA246677 |
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