|
| Status |
Public on Feb 23, 2015 |
| Title |
Ex vivo effects of Tenofovir on four vaginal epithelial cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In MTN-007, a phase 1, randomized, double-blinded rectal microbicide trial, we used systems genomics/proteomics to determine the effect of tenofovir 1% gel, nonoxynol-9 2% gel, placebo gel or no treatment on rectal biopsies taken at baseline, after one application or after seven daily applications (15 subjects/arm). Experiments were repeated using primary vaginal epithelial cells from four healthy women.
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| |
|
| Overall design |
36 Arrays (3 primary vaginal epithelial cell lines from healthy women donors, cultured with or without 50uM or 500 uM Tenofovir for 1,4,7, or 14 days per donor cell line. A no treatment, culture media only control was included for each donor cell line and for each time point.
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| |
|
| Contributor(s) |
Hladik F, Ballweber L, McElrath MJ, McGowan I |
| Citation(s) |
25647729 |
| |
| Submission date |
Apr 23, 2014 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Lamar Ballweber Fleming |
| E-mail(s) |
lballweb@fhcrc.org
|
| Organization name |
Fred Hutchinson Cancer Res Ctr
|
| Department |
VIDD
|
| Lab |
McElrath
|
| Street address |
1100 Fairview Ave N
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98034 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (36)
|
|
| Relations |
| BioProject |
PRJNA245232 |
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