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| Status |
Public on Dec 23, 2014 |
| Title |
Whole transcriptome analysis of FACS purified somatosensory neuron subtypes and whole dorsal root ganglia tissue |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The goal of this study was to analyze global gene expression in specific populations of somatosensory neurons in the periphery, including major, non-overlapping populations that include nociceptors, pruriceptors, and prorioceptors. The mammalian somatosensory nervous system encodes the perception of specific environmental stimuli. The dorsal root ganglion (DRG) contains distinct somatosensory neuron subtypes that innervate diverse peripheral tissues, mediating the detection of thermal, mechanical, proprioceptive, pruriceptive, and nociceptive stimuli. We purified discrete subtypes of mouse DRG somatosensory neurons by flow cytometry using fluorescently labeled mouse lines (SNS-Cre/TdTomato, Parv-Cre/TdTomato) in combination with Isolectin B4-FITC surface staining (IB4). This allowed identification of transcriptional differences between these major populations, revealing enrichment of voltage-gated ion channels, TRP channels, G-protein coupled receptors, transcription factors, and other functionally important classes of genes within specific somatosensory neuron subsets.
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| Overall design |
SNS-Cre mice were bred with Rosa26-TdTomato mice to generate SNS-Cre/TdTomato reporter mice. Parv-Cre mice were bred with Rosa26-TdTomato mice to generate Parv-Cre/TdTomato mice. Isolectin B4-FITC was used to stain the surface of SNS-Cre/TdTomato reporter mice. We used these strategies of fluorescent labeling to purify distinct murine sensory neuron subsets from the dorsal root ganglia (DRG) by fluorescence activated cell sorting (FACS). Neurons were sorted directly in Qiazol for total RNA extraction and microarray analysis. Whole DRG tissue was also included for transcriptome analysis to compare with purified neuronal populations.
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| Contributor(s) |
Chiu IM, Woolf CJ |
| Citation(s) |
25525749 |
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| Submission date |
Feb 18, 2014 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Isaac M Chiu |
| Organization name |
Boston Children's Hospital
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| Department |
Neurology
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| Street address |
3 Blackfan Circle
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (17)
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| GSM1503538 |
SNS-Cre/TdTomato+ DRG neurons, biological replicate 1 |
| GSM1503539 |
SNS-Cre/TdTomato+ DRG neurons, biological replicate 2 |
| GSM1503540 |
SNS-Cre/TdTomato+ DRG neurons, biological replicate 3 |
| GSM1503541 |
SNS-Cre/TdTomato+ DRG neurons, biological replicate 4 |
| GSM1503542 |
Parv-Cre/TdTomato+ DRG neurons, biological replicate 1 |
| GSM1503543 |
Parv-Cre/TdTomato+ DRG neurons, biological replicate 2 |
| GSM1503544 |
Parv-Cre/TdTomato+ DRG neurons, biological replicate 3 |
| GSM1503545 |
Parv-Cre/TdTomato+ DRG neurons, biological replicate 4 |
| GSM1503546 |
Isolectin B4 negative SNS-Cre/Tdtomato+ DRG neurons, biological replicate 1 |
| GSM1503547 |
Isolectin B4 negative SNS-Cre/Tdtomato+ DRG neurons, biological replicate 2 |
| GSM1503548 |
Isolectin B4 negative SNS-Cre/Tdtomato+ DRG neurons, biological replicate 3 |
| GSM1503549 |
Isolectin B4 positive SNS-Cre/Tdtomato+ DRG neurons, biological replicate 1 |
| GSM1503550 |
Isolectin B4 positive SNS-Cre/Tdtomato+ DRG neurons, biological replicate 2 |
| GSM1503551 |
Isolectin B4 positive SNS-Cre/Tdtomato+ DRG neurons, biological replicate 3 |
| GSM1503552 |
Whole DRG tissue, biological replicate 1 |
| GSM1503553 |
Whole DRG tissue, biological replicate 2 |
| GSM1503554 |
Whole DRG tissue, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA238575 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE55114_RAW.tar |
74.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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