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| Status |
Public on Jun 01, 2014 |
| Title |
Analysis of global changes in gene expression induced by human polynucleotide phosphorylase (hPNPaseold-35) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Human Polynucleotide Phosphorylase (hPNPaseold-35) is an evolutionarily conserved 3’→5’ exoribonuclease implicated in the regulation of numerous physiological processes like maintenance of mitochondrial homeostasis, mtRNA import and aging-associated inflammation.
From an RNase perspective, little is known about the RNA or miRNA species it targets for degradation or whose expression it regulates; except for c-myc and miR-221.
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| Overall design |
To further elucidate the functional implications of hPNPaseold-35 in cellular physiology, we knocked-down and overexpressed hPNPaseold-35 in melanoma and HeLa cells, resectively, and performed gene expression analyses to identify differentially expressed transcripts. Biological triplicates were run on microarrays.
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| Contributor(s) |
Fisher P, Dumur CI, Sokhi UK |
| Citation(s) |
24729470 |
| Submission date |
Dec 23, 2013 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Catherine Dumur |
| Organization name |
Sonic Healthcare USA
|
| Department |
Anatomic Pathology
|
| Lab |
Bernhardt
|
| Street address |
5008 Mustang Rd
|
| City |
Jacksonville |
| State/province |
FL |
| ZIP/Postal code |
32216 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA232478 |
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