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| Status |
Public on Nov 21, 2014 |
| Title |
The S-adenosyl-homosteine hydrolase inhibitor DZNeP unspecifically affects histone methylation patterns in embryos and results in developmental aberrations |
| Organism |
Danio rerio |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
3-deazaneplanocin A (DZNeP) is a promising cancer drug affecting the methylation status of histone lysine residues. To investigate the specificity and mode of action of DZNeP, zebrafish embryos displaying high histone methyltransferase activity were cultured with DZNeP and histone methylation (H3K4me3, H3K9me3, H3K27me3) was mapped by ChIP-chip genome-wide promoter at post-MBT stage (5.3 hpf) . We used a custom 2.1M probe HD promoter array (Nimblegen) for ChIP and input DNA hybridization. Peak detection was done using MA2C with P=10e-4 as cutoff.
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| Overall design |
ChIP-chip experiments were performed from chromatin prepared by sonication after formaldehyde cross-linking, from embryos are the indicated developmental stages and ChIP DNA was hybridized onto the aforementioned Nimbegen promoter arrays.
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| Contributor(s) |
Østrup O, Reiner AH, Alestrom P, Collas P |
| Citation(s) |
25260724 |
| |
| Submission date |
Dec 11, 2013 |
| Last update date |
Nov 22, 2014 |
| Contact name |
Philippe Collas |
| Organization name |
University of Oslo
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| Department |
Institute of Basic Medical Sciences
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| Street address |
PO Box 1112 Blindern
|
| City |
Oslo |
| ZIP/Postal code |
0317 |
| Country |
Norway |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA231116 |
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