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| Status |
Public on Jul 19, 2013 |
| Title |
Gene expression analysis of metastatic and non-metastatic osteosarcoma cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Analysis of two metastatic OS cell lines, KHOS and KRIB, and two non-metastatic OS cell lines, HOS and U2OS. Results show differences in gene expression between cell lines with different ability to metastasise in vivo.
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| Overall design |
Each of the four cell lines were analyzed in tripicate. Total RNA was extracted from each triplicate cell line culture with Trizol. cRNA was amplified using the Ambion Illumina Total Prep RNA Amplification Kit. Concentration was determined in NanoDrop and qulity checked in the Agilent Bioanalyzer, before hybridizing to Illumina HT-12 BeadChip Array.
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| |
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| Contributor(s) |
Endo-Munoz L, Saunders NA |
| Citation(s) |
26317203 |
| |
| Submission date |
Jul 18, 2013 |
| Last update date |
Jul 31, 2019 |
| Contact name |
Liliana Beatriz Endo-Munoz |
| E-mail(s) |
l.munoz@uq.edu.au
|
| Phone |
+61734436929
|
| Organization name |
University of Queensland
|
| Department |
Diamantina Institute
|
| Lab |
Saunders
|
| Street address |
TRI, 37 Kent St
|
| City |
Woolloongabba |
| State/province |
Queensland |
| ZIP/Postal code |
4102 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA212559 |
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