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Status |
Public on Jan 30, 2013 |
Title |
SW1 mutant vs. Shewanella piezotolerans WP3 wild-type strain |
Organism |
Shewanella piezotolerans WP3 |
Experiment type |
Expression profiling by array
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Summary |
The prophage and RF DNA of SW1 were removed from WP3 to construct the phage-free strain. The two strains were cultured at 4°C and the transcriptional profiles were compared.
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Overall design |
WP3ΔSW1 is the test sample and WP3 wild-type is the reference sample. Three independent cultures were used for RNA extraction, and the RNA samples were combined into one sample for microarray. Each microarray slide has 3 spots for a single gene, and as dye-swaps were performed, 6 spots were used to determine the expression level of one gene.
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Contributor(s) |
Jian H, Wang F |
Citation(s) |
24038680 |
Submission date |
Jan 29, 2013 |
Last update date |
Jun 06, 2016 |
Contact name |
Huahua Jian |
E-mail(s) |
jiandy@sjtu.edu.cn
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Organization name |
Shanghai Jiao Tong University
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Street address |
No.800 Dongchuan Road
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City |
Shanghai |
ZIP/Postal code |
200240 |
Country |
China |
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Platforms (1) |
GPL16568 |
Shewanella piezotolerans WP3 whole genome microarray |
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Samples (2) |
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Relations |
BioProject |
PRJNA187998 |