|
| Status |
Public on Apr 01, 2014 |
| Title |
The enhancer and promoter landscape of human regulatory and conventional T cell subpopulations |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
CD4+CD25+FOXP3+ human regulatory T cells (Treg) are essential for self-tolerance and immune homeostasis. Here, we generated genome-wide maps of poised and active enhancer elements marked by histone H3 lysine 4 monomethylation and histone H3 lysine 27 acetylation for CD4+CD25highCD45RA+ naive and CD4+CD25highCD45RA- memory Treg and their CD25- conventional T cell (Tconv) counterparts after in vitro expansion . In addition we generated genome-wide maps of the transcription factors STAT5, FOXP3, RUNX1 and ETS1 in expanded CD4+CD25highCD45RA+ Treg- and CD4+CD25- Tconv to elucidate their role in cell type-specific gene regulation.
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| |
|
| Overall design |
ChIP-seq of 2 histone marks and transcription factors ETS1, STAT5, FOXP3 and RUNX1 in expanded T cell subpopulations
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| |
|
| Contributor(s) |
Schmidl C, Rehli M |
| Citation(s) |
24671953 |
| |
| Submission date |
Dec 21, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Michael Rehli |
| E-mail(s) |
michael.rehli@klinik.uni-r.de
|
| Organization name |
University Hospital Regensburg
|
| Department |
Internal Med III
|
| Street address |
F.-J.-Strauss-Allee 11
|
| City |
Regensburg |
| ZIP/Postal code |
93042 |
| Country |
Germany |
| |
|
| Platforms (3) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
| GPL15433 |
Illumina HiSeq 1000 (Homo sapiens) |
| GPL15456 |
Illumina HiScanSQ (Homo sapiens) |
|
| Samples (41)
|
|
| Relations |
| SRA |
SRP017669 |
| BioProject |
PRJNA185269 |
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