|
| Status |
Public on Sep 11, 2012 |
| Title |
Expression data of Schizosaccharomyces pombe deletions involved in DNA damage response |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by array
|
| Summary |
DNA damage response (DDR) plays pivotal roles in maintaining genome integrity and stability. An effective DDR requires the involvement of hundreds of genes that compose a complicated network. To identify novel genes involved in DDR, we screened a genome-wide Schizosaccharomyces pombe (S. pombe) haploid deletion library against six different DNA damage reagents. We identified 52 genes that were actively involved in DDR. Among the 52 genes, 20 genes were linked to DDR for the first time.
For a better understanding of DDR genes function, we performed a DNA microarray assay to analyze the gene expression profiles of eight deletions.
|
| |
|
| Overall design |
Cells of wild type and eight deletions involved in DDR were collected during the expontentially growing stage, and were subjected to RNA extraction and hybridization on Affymetrix microarrays. We tried to understand the role of genes in DDR by comparing the expression profiles of deletions with that of wild type.
|
| |
|
| Contributor(s) |
Pan X, Lu H |
| Citation(s) |
23173672 |
| |
| Submission date |
Sep 10, 2012 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Wenjuan Mo |
| E-mail(s) |
041019012@fudan.edu.cn
|
| Phone |
021-65100243
|
| Fax |
021-65100243
|
| Organization name |
Fudan University
|
| Department |
Life School
|
| Lab |
Genetics national lab
|
| Street address |
No. 220 Handan Road
|
| City |
Shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA174803 |
Less...
More...