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| Status |
Public on Jun 15, 2013 |
| Title |
Transcriptional profiling of gastric epithelial cells infected with wild type or arginase-deficient Helicobacter pylori |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We studied the differential gene expression in the gastric cell line AGS infected with wild type (WT) or arginase gene mutant (rocF) Helicobacter pylori (Hp). We found that the presence of the gene regulates the inflammatory responses in terms of IL8 and MIP-1B. We conclude that arginase may be involved in regulating inflammatory responses which improves the chances of survival against the human immune response.
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| Overall design |
AGS cells were infected for 16 hours with WT, the rocF, or a complemented H. pylori strain (rocF+). The RNA was extracted with TRIzol, treated with DNAse I (both from invitrogen). cRNA was made with the Illumina TotalPrep RNA amplification Kit (ambion AMIL1791).
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| Contributor(s) |
Kim SH, Sierra RA, McGee DJ, Zabaleta J |
| Citation(s) |
22889111 |
| Submission date |
Aug 06, 2012 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Jovanny Zabaleta |
| E-mail(s) |
jzabal@lsuhsc.edu
|
| Phone |
504-210 2979
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| Organization name |
Louisiana State University Health Sciences Center
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| Department |
Cancer Center/Interdiscipliary Oncology
|
| Lab |
LCRC 909
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| Street address |
1700 Tulane Ave.
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| City |
New Orleans |
| State/province |
LA |
| ZIP/Postal code |
70112 |
| Country |
USA |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA172026 |
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