|
| Status |
Public on Dec 21, 2012 |
| Title |
Design and use of multiplexed chemostat arrays |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
Genome variation profiling by array
|
| Summary |
We developed and validated a small-footprint array of miniature chemostats built from readily available parts for low cost. Physiological and experimental evolution results were similar to larger volume chemostats. The ministat array provides a compact, inexpensive, and accessible platform for traditional chemostat experiments, functional genomics, and chemical screening applications.
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| |
|
| Overall design |
Three experiments are gene expression comparisons between three ministat cultures and a single Sixfors sample. The four CGH arrays are individual clones evolved in four sulfate limitation ministats compared to a wt ancestor strain.
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| |
|
| Contributor(s) |
Miller AW, Befort C, Kerr EO, Dunham MJ |
| Citation(s) |
23462663 |
| |
| Submission date |
Mar 21, 2012 |
| Last update date |
Feb 15, 2018 |
| Contact name |
Maitreya J. Dunham |
| E-mail(s) |
maitreya@uw.edu
|
| Phone |
206-543-2338
|
| Organization name |
University of Washington
|
| Department |
Genome Sciences
|
| Lab |
Dunham Lab
|
| Street address |
Foege Building, S403B, Box 355065
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195-5065 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL4131 |
Agilent-014810 Yeast Whole Genome ChIP-on-Chip Microarray 4x44K (G4493A) |
| GPL10045 |
Agilent Yeast 8X15K conversion (Agilent-016322) |
|
| Samples (7)
|
| GSM898829 |
Ministat vs sixfor sulfate limitation RNA replicate 1 |
| GSM898830 |
Ministat vs sixfor sulfate limitation RNA replicate 2 |
| GSM898831 |
Ministat vs sixfor sulfate limitation RNA replicate 3 |
|
| Relations |
| BioProject |
PRJNA153611 |
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