|
| Status |
Public on Dec 21, 2012 |
| Title |
Polycomb Cbx orthologs mediate the balance between Hematopoietic Stem Cell self-renewal and differentiation |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
By genome-wide ChIP-seq analyses we show that Cbx7 and Cbx8 share ~95% of their targets in bone marrow cells.
Cbx7 and Cbx8 have only ~200 targets that are not overlapping between the two proteins.
These distinct Cbx7 and Cbx8 targets show reciprocal expression patterns along hematopoietic differentiation.
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| |
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| Overall design |
Overexpression of empty vector (negative control), flag-Cbx7 and flag-Cbx8 in 5-FU treated bone marrow cells (progenitor and stem cells), followed by 1 week culture and subsequent ChIP using flag-M2 agarose beads.
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| |
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| Contributor(s) |
Klauke K, Radulovic V, Broekhuis MJ, Weersing E, Zwart E, Olthof SG, Ritsema M, Wu X, Helin K, Bystrykh LV, de Haan G |
| Citation(s) |
23502315 |
| |
| Submission date |
Mar 21, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Leonid Bystrykh |
| E-mail(s) |
l.bystrykh@umcg.nl
|
| Organization name |
University Medical Center Groningen
|
| Department |
European Research Institute for the Biology of Ageing - ERIBA
|
| Lab |
Ageing Biology and Stem Cells
|
| Street address |
A. Deusinglaan 1
|
| City |
Groningen |
| State/province |
Groningen |
| ZIP/Postal code |
9713 AV |
| Country |
Netherlands |
| |
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| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
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| Samples (3) |
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| Relations |
| SRA |
SRP011640 |
| BioProject |
PRJNA153665 |
